eBioscience™ Mouse TNF alpha, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8321-85

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Product Code. 15518786

Quantity Price
1 £ 959.3 / 500µg
Estimated Shipment date
from Supplier 31-05-2017
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Description and Specification


Name TNF alpha
Purification Method SDS-PAGE
Conjugate Unlabeled
Quantity 500μg
Cross Reactivity Mouse
Recombinant Yes
Accession Number NM_013693
Regulatory Status RUO
Source E. coli
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
Product Type Recombinant Protein, Carrier-Free
Storage Requirements Store at less than or equal to -70°C.
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Concentration 0.5mg/mL
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 0.22μm filtered.
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693
Purity >98%

Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.