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Invitrogen™ IL-2 Mouse ELISA Kit, High Sensitivity

High Sensitivity Sandwich ELISA Kit

Brand:  Invitrogen™ BMS601HS

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15511987

  • £576.00 / 96 tests
Estimated Shipment: 22-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-2
Biotin-Conjugate anti-mouse IL-2 monoclonal antibody
Streptavidin-HRP
Mouse IL-2 Standard lyophilized, 2000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution
Stop Solution (1M Phosphoric acid)
Adhesive Films

Beschreibung

Beschreibung

The Mouse High Sensitivity Interleukin 2 (HS IL-2) ELISA quantitates Ms HS IL-2 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HS IL-2. Principle of the method The Mouse HS IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
Spezifikation

Spezifikation

P04351
0.05 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
IL-2
3.7%
HRP
RUO
2°C to 8°C
Mouse
4 hr.
0.78-50 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
16183
1.8%
Pre-coated 96 well plate, Standard, Stop Solution, Assay Buffer concentrate, Standard, Sample Diluent, Amplification Reagent I, Amplification Reagent II, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Aldesleukin, T cell growth factor, TCGF
1 hr. 20 min.
Produktvorschläge

Produktvorschläge

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Dokumentation

Dokumentation

Produktzertifizierungen
Sonderangebote

Sonderangebote

For Research Use Only