Invitrogen™ Mouse IL-2 ELISA Kits

Measure levels of mouse interleukin-2 in mouse serum and cell culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EMIL2

Product Code. 10300974

Description

Assay IL-2 (mouse interleukin-2) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-2 ELISA Kits.

These mouse IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant mouse interleukin-2 (IL-2)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 34 to 850pg/mL

• Sensitivity: < 3pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, serum

• Total Assay Time: 3.5 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with mouse IL-3, IL-4,IL-5, IL-6, IL-10, IFNγ, GM-CSF, TNFα or human IL-2

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

• Assay Range: 34 to 850pg/mL
• Description: Mouse IL-2 ELISA Kit
• Sample Type: Cell Culture Supernatant, Serum
• Sufficient For: 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 1 Plate Kit
• Target: Natural and recombinant mouse interleukin-2 (IL-2)

• Assay Sensitivity: <3pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with mouse IL-3, IL-4,IL-5, IL-6, IL-10, IFNg, GM-CSF, TNFa or human IL-2
• Kit Contents: TMB Substrate Solution, 13mL, Stop Solution, 13mL, Anti-Mouse IL-2 Precoated 96-well Strip Plate, 1 ea, Standard Diluent, 12mL, Plate Reagent, 12mL, Conjugate Dilution Buffer, 14mL, Anti-IL2-HRP Conjugate Concentrate, 0.10mL, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, Lyophilized Recombinant Mouse IL-2 Standard, 2 vials
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Sample Volume: 50 μL
• Total Assay Time: 3.5 hr.