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Invitrogen™ IL-1 alpha Mouse ELISA Kit

For the quantitative detection of mouse IL-1alpha.

Brand:  Invitrogen™ BMS611

300.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15592027

  • £451.00 / 96 tests
Estimated Shipment: 22-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-1alpha
Biotin-Conjugate anti-mouse IL-1alpha monoclonal antibody
Streptavidin-HRP
Mouse IL-1alpha Standard lyophilized, 1000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Mouse Interleukin-1alpha (Ms IL-1α) ELISA quantitates Ms IL-1α in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-1α. Principle of the method The Mouse IL-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Specifications

Specifications

P01582
4.0 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
Il-1a
5%
HRP
RUO
2°C to 8°C
Mouse
3 hr. 10 min.
7.8-500 pg/mL
Biotin
Serum, Supernatant
ELISA
16175
10%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Serum, 50 μL; Supernatant, 50 μL
Hematopoietin 1, IL1F1, Interleukin 1 alpha
1 hr. 20 min.
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