Invitrogen™ IL-17F (Homodimer) Mouse Uncoated ELISA Kit

Specifically engineered for accurate and precise measurement of mouse IL-17FF homodimer protein levels from samples including serum, and cell culture supernatants.

Brand:  Invitrogen™ 88-7472-86

Product Code. 15521157

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

The Mouse Interleukin 17F (Homodimer) (IL-17F) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17F. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Mouse IL-17F ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)

• The assay demonstrates parallelism in measuring recombinant and native mouse IL-17F proteins and a standard curve range of 15 to 2000pg/mL
• No crossreactivity to the IL-17AA homodimer is observed and <2.5% crossreactivity to IL-17AF heterodimer when spiked in excess (100ng/ml)

Specifications

• Assay Sensitivity: 15pg/mL
• For Use With (Application): ELISA
• Storage Requirements: 2°C to 8°C except standard which should be stored at ≤-70°C
• Target: IL-17F

• Target Species: Mouse
• Quantity: 10 x 96 Tests
• Synonym: Interleukin-17F, IL-17FF

For Research Use Only