Invitrogen™ IL-25/IL-17E Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS6046TEN

Product Code. 15592007

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-17E (IL-25)
Biotin-Conjugate anti-mouse IL-17E (IL-25) monoclonal antibody
Streptavidin-HRP
Mouse IL-17E (IL-25) Standard lyophilized, 500pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

The Mouse Interleukin-25/Interleukin 17E (Ms IL-25/IL-17E) ELISA quantitates Ms IL-25/IL-17E in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-25/IL-17E. Principle of the method The Mouse IL-25/IL-17E solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Assay Sensitivity: 0.9pg/mL
• Target Species: Mouse
• Quantity: 10 x 96 Tests
• Target: IL-17E (IL-25)

• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate), Serum
• For Use With (Application): ELISA
• Synonym: Interleukin-17E, Interleukin-25