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Invitrogen™ Human BAFF (BLyS) Recombinant Protein, eBioscience™
Recombinant Protein
Brand: Invitrogen™ 14-8943-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified. Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity. Applications Reported: Recombinant human BAFF is biologically active. Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6ug/mL. Source: E. coli expressed amino acids Ala134-Leu285 accession &Num; Q9Y275. Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6ug/mL. Endotoxin: Less than 0.40ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE. Molecular Weight: 18.5 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2um post-manufacturing filtered.Specifications
Q9Y275 | |
Control, Functional Assay | |
10673 | |
Human CD257 (BAFF, BLyS) | |
25μg | |
-80°C | |
E. coli expressed amino acids Ala134 - Leu285 Accession # Q9Y275 | |
Human CD257 (BAFF, BLyS) | |
The ED50, as determined by a mouse splenocyte survival assay, is 1.6μg/mL. | |
Human | |
Hu | |
Recombinant | |
Recombinant |
0.1mg/mL | |
PBS with no preservative | |
18.5kDa | |
reducing SDS-PAGE | |
E. coli | |
RUO | |
Less than 0.40ng/μg as determined by the LAL assay. | |
TNFSF13B | |
Recombinant Protein | |
Unconjugated | |
E. coli | |
Liquid | |
Affinity chromatography |