Mouse M-CSF, Invitrogen™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8983-62

Product Code. 15537556

Description

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Description: Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Applications Reported: Recombinant mouse M-CSF is biologically active. Applications Tested: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Source: Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530. Bioactivity: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 37.79 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Accession Number: P07141
• For Use With (Application): Control, Functional Assay
• Gene ID (Entrez): 12977
• Name: Mouse M-CSF
• Purification Method: SDS-PAGE
• Source: Insect Cells
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Csf1
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Baculovirus
• Form: Liquid
• Purity or Quality Grade: Affinity chromatography

• Concentration: 0.1mg/mL
• Formulation: PBS with 1% BSA and no preservative; pH 7.2
• Molecular Weight (g/mol): 37.79kDa
• pH Range: 7.2
• Quantity: 5μg
• Storage Requirements: -80°C
• Structural Form: Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530
• Common Name: Mouse M-CSF
• Biological Activity: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10⁵ Units/mg.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant