eBioscience™ Mouse M-CSF, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 14-8983-62

5UG Mouse M-CSF Recombinant Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15537556

Quantity Price
1 £ 46.6 / 5µg
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

Accession Number NM_001113530
Concentration 0.1mg/mL
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid; phosphate buffered saline with 1% BSA, pH 7.2 0.22μm filtered.
Molecular Weight 37.79kDa
Name M-CSF
Purification Method SDS-PAGE
Purity >98%
Quantity 5μg
Recombinant Yes
Source Insect Cells
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Structural Form Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Gene Symbol Macrophage colony stimulating factor, monocyte colony stimulating factor
Biological Activity The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 105 Units/mg.
Product Type Recombinant Protein

Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization.

M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex.