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Invitrogen™ IL-13 Mouse ELISA Kit
For the quantitative detection of mouse IL-13.
Brand: Invitrogen™ BMS6015TEN
1600.08 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : NEW
Additional Details : Weight : 0.23750kg
Includes: Capture Antibody Concentrate (250 x)
Detection Antibody Concentrate (250 x)
mouse IL-19 standard, (lyophilized), 8000pg/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS) 10x
Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent Z
Tetramethylbenzidine (TMB) Substrate Solution
2 96-well plates
Description
The Mouse Interleukin-13 (Ms IL-13) ELISA quantitates Ms IL-13 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-13. Principle of the method The Mouse IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.Specifications
P20109 | |
2.8 pg/mL | |
ELISA Kit | |
Mouse | |
Colorimetric Microplate Reader | |
IL-13 | |
4.6% | |
HRP | |
RUO | |
2°C to 8°C | |
Mouse | |
3 hr. 10 min. |
7.8-500 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
16163 | |
5% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
10 x 96 Tests | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
ALRH, BHR1, allergic rhinitis | |
1 hr. 20 min. |
For Research Use Only