missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Mouse IL-12 (total) ELISA Kits

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EMIL12TOT

Code : PN

Additional Details : Weight : 0.10000kg

Product Code. 10485335

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 47 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

47 to 3000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Mouse IL-12 Total Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, HRP Dilution Buffer, 13mL, Lyophilized Recombinant Mouse IL-12 Standard, 2 vials, Standard Diluent, 20mL, Stop Solution, 13mL, Streptavidin-HRP Concentrate, 75uL, TMB Substrate Solution, 12mL
Absorbance at 450nm minus absorbance at 550nm
<12pg/mL
50µL
96 microplate wells
Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Mouse IL-12 Total (p70, p40) ELISA Kit
Complete ELISA kit with coated 96-well plate
Dependent upon sample type (generally, intra- and inter-assay CV <10%)
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
3 hr.
Videos
Documentation

Technical Documents