eBioscience™ Mouse IL-12, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 14-8121-62

5UG Mouse IL-12 p70 Recombinant Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15577496

Quantity Price
1 £ 127.8 / 5µg
EU Stock 2
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Description and Specification

Specification

For Use With (Application) Cytokine Bioassays, ELISA Recombinant Standard
Molecular Weight The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65kDa protein. The DTT reduced protein migrates as 43kDa and 24kDa polypeptides.
Regulatory Status RUO
Name IL-12
Purification Method SDS-PAGE
Accession Number NM_008352 co-expressed with NM_008351
Purity >98%
Quantity 5μg
Recombinant Yes
Source Insect cells infected with baculovirus
Structural Form Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, accession # NM_008352 was co-expressed with mouse p35, amino acids met 1-ala 215, accession # NM_008351
Endotoxin Concentration Less than 0.01ng/μg cytokine, as determined by the LAL assay.
Gene Symbol Interleukin-12, IL12, p70
Validated Application ELISA, FA
Conjugate Unlabeled
Biological Activity The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 106 Units/mg.
Cross Reactivity Mouse
Formulation Sterile liquid; 10mM NaH2PO4, pH 6.0, 300mM NaCl, with 1.0% BSA. 0.22μm filtered.

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience’s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.