eBioscience™ Mouse IL-12, Recombinant Protein

Mouse Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8121-62

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15577496

Quantity Price
1 £ 127.8 / 5µg
EU Stock 4
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Description and Specification


For Use With (Application) Cytokine Bioassays, ELISA Recombinant Standard
Molecular Weight The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65kDa protein. The DTT reduced protein migrates as 43kDa and 24kDa polypeptides.
Regulatory Status RUO
Name IL-12
Purification Method SDS-PAGE
Quantity 5μg
Purity >98%
Conjugate Unlabeled
Recombinant Yes
Biological Activity The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 106 Units/mg.
Structural Form Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, accession # NM_008352 was co-expressed with mouse p35, amino acids met 1-ala 215, accession # NM_008351
Accession Number NM_008352 co-expressed with NM_008351
Source Insect cells infected with baculovirus
Formulation Sterile liquid; 10mM NaH2PO4, pH 6.0, 300mM NaCl, with 1.0% BSA. 0.22μm filtered.
Endotoxin Concentration Less than 0.01ng/μg cytokine, as determined by the LAL assay.
Gene Symbol Interleukin-12, IL12, p70
Cross Reactivity Mouse
Validated Application ELISA, FA

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience’s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.