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Thermo Scientific™ Pierce™ Mouse IL-10 ELISA Kits

Measure levels of mouse interleukin-10 in mouse serum and cell culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EM2IL10

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.45000kg Transport : UN number : 3261 Chem class : 1a Pack group : III

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Product Code. 10065733

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Description

Description

These mouse IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 37 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, IFNγ, TNFα or human IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

37 to 3000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL
Absorbance at 450nm minus absorbance at 550nm
<12pg/mL
50µL
96 microplate wells
Natural and recombinant mouse interleukin-10 (IL-10)
Mouse IL-10 ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, serum
No significant cross-reaction with mouse IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, IFNg, TNFa or human IL-10
5 hr.
Documentation

Technical Documents

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