Invitrogen™ Mouse IL-1 alpha ELISA Kits

Measure levels of mouse interleukin-1 alpha (IL-1a) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EMIL1A

Product Code. 10209193

Description

Assay IL-1aα (mouse interleukin-1 alpha) levels in mouse serum, plasma (EDTA, heparin, sodium citrate)and culture supernatant samples with our Thermo Scientific Mouse IL-1 alpha ELISA Kits.

These mouse IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant mouse interleukin-1 alpha (IL-1α)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 15.6 to 1000pg/mL

• Sensitivity: < 6pg/mL

• Precision: Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Assay Range: 15.6 to 1000pg/mL
• Description: Mouse IL-1 alpha ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Sufficient For: 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 1 Plate Kit
• Target: Natural and recombinant mouse interleukin-1 alpha (IL-1a)

• Assay Sensitivity: <6pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with human IL-1a, human IL-1ß, or mouse IL-1ß, IL-3, IL-4, IL-5, IL-7, IL-10, TNFa, IFNg, or GM-CSF
• Kit Contents: Biotinylated Antibody Reagent, 8mL, Anti-Mouse IL-1 alpha Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, HRP Dilution Buffer, 14mL, Streptavidin-HRP Concentrate, 75µL, Lyophilized Recombinant Mouse IL-1 alpha Standard, 2 vials, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Standard Diluent, 12mL
• Precision: Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%
• Sample Volume: 50 μL
• Total Assay Time: 3 hr.