Thermo Scientific™ Pierce™ Mouse IL-1 alpha ELISA Kits

Measure levels of mouse interleukin-1 alpha (IL-1a) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: EMIL1A2

MOUSE IL-1A ELISA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10571004

Quantity Price
1 £ 443.0 / 1 set
Estimated Shipment date
from Supplier 20-12-2016
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Description and Specification

Specification

Assay Range 15.6 to 1000pg/mL
Description Mouse IL-1 alpha ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 2 x 50mL
Precision Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 2 plate kit
Sensitivity <6pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with human IL-1a, human IL-1ß, or mouse IL-1ß, IL-3, IL-4, IL-5, IL-7, IL-10, TNFa, IFNg, or GM-CSF
Sufficient For 2 x 96 microplate wells
Total Assay Time 3 hr.
Target Natural and recombinant mouse interleukin-1 alpha (IL-1a)

These mouse IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-1 alpha (IL-1α)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.6 to 1000pg/mL

  • Sensitivity: < 6pg/mL

  • Precision: Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Documentation

Technical documents