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Invitrogen™ IgG1 Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ 88-50410-77

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15590877

  • £434.00 / Pack of 20


In stock, ready to ship: 1
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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Mouse IgG1 isotype control (standard), lyophilized, 400ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)

Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

Colorimetric Microplate Reader
3.13-200 ng/mL
ELISA
ELISA Kit
3.13 ng/mL
Plasma, 10 μL|Serum, 10 μL|Supernatant, 50 μL
1 hr. 45 min.
Mouse
20 x 96 Tests
Supernatant
HRP
HRP
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
RUO
50 μL (prediluted 1:10,000)
2°C to 8°C
24 hr. 30 min.
Immunoglobulin G1
Mouse
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For Research Use Only