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Mouse IgG1 Isotype Control, Invitrogen™

Mouse IgG1 Isotype Control, MA5-18092, from Invitrogen™.

Brand:  Invitrogen MA518092

UNSPSC : 12352200

Code : NEW

Additional Details : Weight : 0.01000kg

Product Code. 15397364

  • £210.00 / 100 tests


Estimated Shipment
08-09-2017
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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody’s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG1
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: 100 TESTS purified isotype control antibody (0.1mg/mL) in PBS with 1% BSA and 0.09% sodium azide
MA5-18092 is negative on all human cells and cell lines tested. This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. Test results have shown that MA5-18092 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Specifications

Specifications

Mouse IgG1
Unlabeled
Purified
Rat thymocyte membrane glycoproteins
Protein A
Store at 4°C or Store at -20°C if preferred
Immunohistochemistry (Frozen)
100 TESTS purified isotype control antibody (0.1mg/mL) in PBS with 1% BSA and 0.09% sodium azide
Murine
IgG1
100 Tests
Not Applicable