Mouse IgG1 Control

Mouse IgG1 Control from Thermo Scientific™ Pierce™ is validated for Ctrl, FACS and IHC (F).

Overview
Brand: Invitrogen

Manufacturer Part Number: MA518167

Control Antibody,Mouse IgG1 Isotype,Alexa Fluor

UNSPSC: 12352203

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15347434

Quantity Price
1 £ 241.0 / 100µg
Estimated Shipment date
from Supplier 22-12-2016
Add to basket


Description and Specification

Specification

Antigen Mouse IgG1
Applications Control
Applications Flow Cytometry
Clone MOPC-21
Conjugate Alexa Fluor 488
Format Purified
Formulation 100μg purified isotype control antibody (1mg/mL) in PBS and 15mM sodium azide; pH 7.4
Host Species Mouse
Isotype IgG1
Purification Method Size-exclusion chromatography
Quantity 100μg
Species Reactivity Not Applicable
Storage Requirements DO NOT FREEZE!

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG1
Clone: MOPC-21
Formulation: 100μg purified isotype control antibody (1mg/mL) in PBS and 15mM sodium azide; pH 7.4
This product can be used as a negative control to measure the non-specific binding of Mouse IgG1 in human and rat tissues; it has been tested on a variety of resting, activated, live and fixed rat and human tissues.