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Invitrogen™ IgE Mouse Uncoated ELISA Kit with Plates

For quantitative detection of mouse IgE.

Brand:  Invitrogen™ 88-50460-86

Code : NEW

Additional Details : Weight : 0.47500kg

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Product Code. 15540907

  • £330.00 / Pack of 10


Estimated Shipment: 06-10-2021
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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
mouse IgE isotype control (standard), lyophilized, 500ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution
96 Well Plate included with product Cat. #’s ending in suffixes -22, -76, -86

Description

Description

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
Specifications

Specifications

HRP
Biotin
ELISA
RUO
2°C to 8°C
24 hr. 30 min.
Immunoglobulin E
Mouse
4 ng/mL
ELISA Kit
4-250 ng/mL
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
Plasma, 10 μL; Serum, 10 μL; Supernatant, 50 μL
1 hr. 45 min.
Mouse
10 x 96 Tests
Supernatant
Colorimetric Microplate Reader
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