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Invitrogen™ Mouse IFN gamma ELISA Kits

Measure levels of mouse interferon gamma (IFNg) in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ ESS0020

Code : PN

Additional Details : CAS Number : 306935-40-0 Weight : 0.10000kg

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Product Code. 10617914

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Description

Description

These mouse IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 37 to 3000pg/mL

  • Sensitivity: < 10pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

37 to 3000pg/mL
Mouse IFN gamma ELISA Reagent Kit
Antibodies and complete reagent set for ELISA
Absorbance at 450nm minus absorbance at 550nm
<10pg/mL
50µL
5 x 96 microplate wells
Natural and recombinant mouse interferon gamma (IFNg)
IFN gamma Biotinylated Detection Antibody, 1 vial
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
5 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with with mouse IL-1a, IL-1ß, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFa or human IFNa or IFNg
4 hr.
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