missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Mouse IFN gamma ELISA Kits

Measure levels of mouse interferon gamma (IFNg) in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EM10012

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

 View more versions of this product

Product Code. 10494984

  • £453.00 / 1 set


Estimated Shipment
12-06-2017
Add to basket

Specifications

Description

These mouse IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 37 to 3000pg/mL

  • Sensitivity: < 10pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Description & Specifications

Specifications

<10pg/mL
Complete ELISA kit with coated 96-well plates
30X Wash Buffer, 2 x 50mL
2 x 96 microplate wells
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Mouse IFN gamma ELISA Kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
4 hr.
Intra-assay CV <10%; inter-assay CV <10%
Absorbance at 450nm minus absorbance at 550nm
37 to 3000pg/mL
No significant cross-reaction with with mouse IL-1a, IL-1ß, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFa or human IFNa or IFNg
50µL
Natural and recombinant mouse interferon gamma (IFNg)
2 plate kit
Documentation

Technical Documents