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Mouse HMGB1 Carrier-Free (High Mobility Group Box 1), Invitrogen™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8401-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15598796

  • £844.00 / 100µg

Estimated Shipment
11-11-2020

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Description

Description

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury. Description: High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. Applications Reported: Mouse HMGB1 Recombinant Protein Carrier-Free is biologically active. Applications Tested: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25ug/mL in the presence of 10ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg. Source: E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession &Num; NM_010439. Bioactivity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25ug/mL in the presence of 10ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

P63158
Control, Functional Assay
Control, Functional Assay
20mM tris HCl with 0.2M NaCl, 1mM DTT and no preservative; pH 8.0
The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6kDa.
8.0
Affinity chromatography
E. coli
RUO
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Hmgb1
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
Recombinant
0.5mg/mL
Liquid
15289
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1)
SDS-PAGE
100μg
-80°C
E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1)
The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25μg/mL in the presence of 10μg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
Mouse
Ms
Recombinant
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