eBioscience™ Mouse TGF beta 1, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8342-62

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15577526

Quantity Price
1 £ 164.47 / 5µg
EU Stock 5
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Description and Specification

Specification

Biological Activity The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40pg/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 107 units/mg.
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Purification Method SDS-PAGE
For Use With (Application) Functional Assays , Cell Culture
Purity >95%
Product Type Recombinant Protein
Formulation Sterile liquid; 0.1M Gly-HCl, pH 3.0, 1% BSA, 0.22 µm
Gene Symbol Transforming growth factor beta-1
Regulatory Status RUO
Storage Requirements Store at less than or equal to -70°C.
Quantity 5μg
Molecular Weight Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein.
Name TGF beta 1
Concentration 0.1mg/mL
Cross Reactivity Mouse
Recombinant Yes
Conjugate Unlabeled

Mouse TGF beta 1 (Transforming Growth Factor beta 1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF beta 1 is a 25 kDa protein with each subunit containing 112 aa residues. TGF beta 1 is secreted from the many cell types in a complex with LAP (Latency Associated Peptide) and also with LTBP (latent TGF beta binding protein). Disassociation is thought to happen in the extracellular matrix via proteases and/or integrins. Regulatory T cells are a recently identified source of TGF beta 1 thought to be a key player in their suppressive functions. TGF beta 1 binds through TGF beta receptors (of which there is a large family). The protein is highly conserved across all mammalian species.