eBioscience™ Mouse TGF beta 1, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 14-8342-62

5UG Mouse TGF beta 1 Recombinant Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15577526

Quantity Price
1 £ 250.6 / 5µg
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

Concentration 0.1mg/mL
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Functional Assays , Cell Culture
Formulation Sterile liquid; 0.1M Gly-HCl, pH 3.0, 1% BSA, 0.22 µm
Molecular Weight Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein.
Name TGF beta 1
Purification Method SDS-PAGE
Purity >95%
Quantity 5μg
Recombinant Yes
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Gene Symbol Transforming growth factor beta-1
Biological Activity The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40pg/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 107 units/mg.
Product Type Recombinant Protein

Mouse TGF beta 1 (Transforming Growth Factor beta 1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF beta 1 is a 25 kDa protein with each subunit containing 112 aa residues. TGF beta 1 is secreted from the many cell types in a complex with LAP (Latency Associated Peptide) and also with LTBP (latent TGF beta binding protein). Disassociation is thought to happen in the extracellular matrix via proteases and/or integrins. Regulatory T cells are a recently identified source of TGF beta 1 thought to be a key player in their suppressive functions. TGF beta 1 binds through TGF beta receptors (of which there is a large family). The protein is highly conserved across all mammalian species.