eBioscience™ Human TPO, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8685-61

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15548816

Quantity Price
1 £ 60.53 / 2µg
Estimated Shipment date
from Supplier 01-06-2017
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Description and Specification

Specification

Name TPO
Biological Activity The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 106 Units/mg.
Regulatory Status RUO
Source E. coli
Recombinant Yes
Quantity 2μg
Gene Symbol THPO, MPL ligand
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Purity >98%
Formulation Lyophilized from 10mM sodium citrate, pH 3.0
Purification Method SDS-PAGE and HPLC
Product Type Recombinant Protein, Carrier-Free
Conjugate Unlabeled
Structural Form E. coli (amino acids Glu22-Gly195; Accession # AAB33390.1)
Molecular Weight 18.6kDa
For Use With (Application) Functional Assays
Cross Reactivity Human

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.