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Human Thrombopoietin (TPO) Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8685-61

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15548816

  • £62.18 / 2µg


Estimated Shipment date
from Supplier 26-07-2018
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Description

Description

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Description: Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Applications Reported: Recombinant human TPO is biologically active and can promote proliferation of human MO7e cells in culture. Applications Tested: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Source: E. coli (amino acids Glu22-Gly195; accession # AAB33390.1). Bioactivity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 18.6 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of less than 0.5mg/mL. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Specifications

Specifications

P40225
Lyophilized
7066
Human Thrombopoietin (TPO) Carrier-Free
SDS-PAGE and HPLC
2μg
-20°C
E. coli (amino acids Glu22-Gly195; Accession # AAB33390.1)
Less than 0.1ng/μg cytokine as determined by the LAL assay
THPO
The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 106 Units/mg.
Human
Hu
Recombinant
Functional Assays
0.01M sodium citrate with no preservative; pH 3.0
18.6kDa
3.0
Affinity chromatography
E. coli
RUO
Recombinant
Human Thrombopoietin (TPO) Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
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