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Thermo Scientific™ Pierce™ Mouse GM-CSF ELISA Kits

Measure levels of granulocyte macrophage colony-stimulating factor in mouse serum and culture supernatants using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EMGMCSF2

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10742905

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Specifications

Description

These mouse GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 0 to 250pg/mL

  • Sensitivity: < 5pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, IFNγ, TNFα, rabbit GM-CSF, rat GM-CSF or human GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

0 to 250pg/mL
Mouse GM-CSF ELISA Kit
Complete ELISA kit with coated 96-well plates
2 plate kit
<5pg/mL
50┬ÁL
2 x 96 microplate wells
Natural and recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF)
Adhesive Plate Covers, 12 ea
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Absorbance at 450nm minus absorbance at 550nm
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Cell culture supernatant, serum
No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, IFNg, TNFa, rabbit GM-CSF, rat GM-CSF or human GM-CSF
3.5 hr.
Documentation

Technical Documents