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eBioscience™ SAFE™ Human GM-CSF, Recombinant Protein

Human Recombinant Protein

Brand:  Affymetrix eBioscience 68-8777-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15500627

  • £360.13 / 100µg


Estimated Shipment date
from Supplier 29-06-2017
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Specifications

Description

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.
Description & Specifications

Specifications

Functional Assays
26kDa with glycosylation
SDS-PAGE
100μg
The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Plant expressed 127 amino acids (Ala18-Glu144). Accession NP_000749.2
Granulocyte Macrophage Colony Stimulating Factor
Recombinant Protein
Human
Sterile lyophilized (PBS buffer pH 7.2)
GM-CSF
≥95%
Plant
RUO
Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Unlabeled
Yes