eBioscience™ SAFE™ Human GM-CSF, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 68-8777-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15500627

Quantity Price
1 £ 360.13 / 100µg
Estimated Shipment date
from Supplier 03-05-2017
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Description and Specification

Specification

Regulatory Status RUO
Recombinant Yes
Quantity 100μg
For Use With (Application) Functional Assays
Purity ≥95%
Gene Symbol Granulocyte Macrophage Colony Stimulating Factor
Endotoxin Concentration Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Name GM-CSF
Structural Form Plant expressed 127 amino acids (Ala18-Glu144). Accession NP_000749.2
Conjugate Unlabeled
Cross Reactivity Human
Formulation Sterile lyophilized (PBS buffer pH 7.2)
Storage Requirements The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Biological Activity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Molecular Weight 26kDa with glycosylation
Purification Method SDS-PAGE
Product Type Recombinant Protein
Source Plant

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.