eBioscience™ SAFE™ Human IL-4, Recombinant Protein

Human Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 68-8780-63

10UG SAFE Human IL-4 Recombinant Animal Free Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15520627

Quantity Price
1 £ 148.1 / 10µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Conjugate Unlabeled
Cross Reactivity Human
For Use With (Application) Functional Assays
Formulation Sterile lyophilized (PBS buffer pH 7.2)
Molecular Weight 17.2kDa (apparent 23 and 24kDa)
Name IL-4
Purification Method SDS-PAGE
Purity ≥95%
Quantity 10μg
Recombinant Yes
Source Plant
Storage Requirements The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Regulatory Status RUO
Structural Form Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. Accession number NP_000580.
Endotoxin Concentration Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Biological Activity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Product Type Recombinant Protein

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.