eBioscience™ SAFE™ Human IL-4, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 68-8780-63

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15520627

Quantity Price
1 £ 97.22 / 10µg
Estimated Shipment date
from Supplier 01-06-2017
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Description and Specification

Specification

For Use With (Application) Functional Assays
Molecular Weight 17.2kDa (apparent 23 and 24kDa)
Endotoxin Concentration Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Recombinant Yes
Purification Method SDS-PAGE
Conjugate Unlabeled
Name IL-4
Regulatory Status RUO
Product Type Recombinant Protein
Source Plant
Purity ≥95%
Biological Activity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Formulation Sterile lyophilized (PBS buffer pH 7.2)
Structural Form Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. Accession number NP_000580.
Cross Reactivity Human
Quantity 10μg
Storage Requirements The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.