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eBioscience™ SAFE™ Human IL-4, Recombinant Protein

Human Recombinant Protein

Brand:  Affymetrix eBioscience 68-8780-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15530627

  • £402.93 / 100µg


Estimated Shipment date
from Supplier 28-07-2017
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Specifications

Description

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.
Description & Specifications

Specifications

Functional Assays
17.2kDa (apparent 23 and 24kDa)
SDS-PAGE
100μg
The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. Accession number NP_000580.
The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Unlabeled
Yes
Sterile lyophilized (PBS buffer pH 7.2)
IL-4
≥95%
Plant
RUO
Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Recombinant Protein
Human