Invitrogen™ TNFR1 (Soluble) Monkey ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS653

Product Code. 15512107

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sTNF-R I
HRP-Conjugate anti-monkey sTNF-R I monoclonal antibody
Monkey sTNF-R I Standard concentrate, 12 U/ml upon dilution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

The Monkey Tumor Necrosis Factor Receptor 1 (Mk TNFR1) ELISA quantitates Mk TNFR1 in monkey(baboon, cynomolgus) serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk TNFR1. Principle of the method The Monkey TNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor 1 (TNFR1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating apoptosis. TNFR1 also plays an important role in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of the receptor is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.

Specifications

• Assay Range: 0.09-6.0 U/mL
• Conjugate: HRP
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 101013835, 102135285
• Interassay CV: 9%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Serum, 10 μL; Supernatant, 10 μL
• Synonym: CD120a, TNFR60, TNF Receptor superfamily member 1A
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 0.06 U/mL
• Product Type: ELISA Kit
• Target Species: Monkey
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: EGK_03268
• Intraassay CV: 2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Baboon, Cynomolgus Monkey
• Total Assay Time: 2 hr. 10 min.

For Research Use Only