missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ CD31/PECAM (Soluble) Monkey ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS651TEN

1870.09 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15502107

  • £2805.00 / Pack of 10
Estimated Shipment: 23-04-2024
to see stock.
Add to basket

Includes: Aluminum pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey sPECAM-1
HRP-Conjugate anti-monkey-sPECAM-1 monoclonal (murine) antibody
Monkey sPECAM-1 Standard
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent (buffered protein matrix)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

The Monkey Soluble CD31/Platelet Endothelial Cell Adhesion Molecule (MK sCD31/PECAM) ELISA quantitates Mk sCD31/PECAM in monkey(baboon) serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk sCD31/PECAM. Principle of the method The Mk sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. The N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. Further, CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. CD31 is one of key regulatory molecules in the vascular system. CD31 induces susceptibility to atherosclerosis and this cell adhesion molecule is required for leukocyte migration under most inflammatory conditions. Antibodies to CD31 is of value in the study of benign and malignant vascular tumors.
Specifications

Specifications

0.48-30.0 U/mL
HRP
Serum, Supernatant
ELISA
101003471
10%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
10 x 96 Tests
Serum, 10 μL; Supernatant, 10 μL
Leukocyte surface protein, CD31, EndoCAM, HEC7
1 hr. 20 min.
0.06 U/mL
ELISA Kit
Monkey
Colorimetric Microplate Reader
PECAM1
2%
HRP
RUO
2°C to 8°C
Baboon
3 hr. 10 min.
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only