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Invitrogen™ P-Selectin (Soluble) Monkey ELISA Kit

For the quantitative detection of monkey sP-selectin.

Brand:  Invitrogen™ BMS650-4TEN

1870.09 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15572097

  • £2805.00 / Pack of 10
Estimated Shipment: 23-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sP-selectin
HRP-Conjugate anti-monkey sP-selectin monoclonal antibody
Monkey sP-selectin Standard lyophilized, 100.0 U/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

The Monkey P-Selectin (CD62) ELISA quantitates Mk P-Selectin in baboon serum or monkey cell culture medium. The assay will exclusively recognize both natural and recombinant Mk P-Selectin. Principle of the method The Monkey P-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
Specifications

Specifications

0.8-50 U/mL
HRP
Serum, Supernatant
ELISA
101009024, 102141875, 701277
10%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
10 x 96 Tests
Serum, 10 μL; Supernatant, 10 μL
Granule membrane protein 140, PADGEM, CD62P
1 hr. 20 min.
1.3 U/mL
ELISA Kit
Monkey
Colorimetric Microplate Reader
SELP
10%
HRP
RUO
2°C to 8°C
Baboon, Cynomolgus Monkey, Rhesus Monkey
2 hr. 30 min.
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