beta Galactosidase Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen A11132

Product Code. 10654773

Description

The E. coli gene lacZ encoding beta-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of beta-galactosidase is not practical, anti-beta-galactosidase antibody has been widely used. Anti-beta-galactosidase antibody can also be used for purification of fusion proteins by immunoprecipitation or immunoadsorption. Furthermore, anti-beta-galactosidase antibody has been shown to be the preferred analytical tool for light microscopic evaluation of lacZ gene transfer, and the beta-galactosidase/anti-beta-galactosidase complex has been used as a model to assess the feasibility of a high-pressure immunodesorption process. This antibody, raised in rabbit against E. coli beta-galactosidase, demonstrates high specificity for the enzyme and is suitable for immunological methods that require high titer and specificity such as immunoblotting (western or dot blot), ELISA, immunoprecipitation, and immunohistochemistry. The appropriate dilution of antibody for a particular application should be determined empirically. We suggest trying dilutions of about 1:5,000 to 1:50,000 for your initial experiments.

beta Galactosidase Polyclonal antibody specifically detects beta Galactosidase in Bacteria samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot.

Order Info

Shipping Condition: Wet Ice

Specifications

• Antigen: beta Galactosidase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; GLB1; Glb-1; lactase; lysosmal hydrolase; lysosomal beta-galactosidase; lysosomal hydrolase; MPS4B; βgal; β-gal; β-galactosidase
• Immunogen: beta-Galactosidase
• Quantity: 500 μL
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Form: Liquid

• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
• Concentration: 2 mg/mL
• Formulation: PBS with 5mM sodium azide; pH 7.2
• Host Species: Rabbit
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

For Research Use Only. Not for use in diagnostic procedures.