Invitrogen™ EdU (5-ethynyl-2’-deoxyuridine)

EdU can be used as a replacement for BrdU and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry.

Brand:  Invitrogen™ E10187

Product Code. 10617215

Description

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS).

EdU is readily soluble in DMSO, alcohol, water, or aqueous buffers. For use with in vitro applications, prepare 10 mM EdU stock solution in DMSO or aqueous buffer.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Order Info

Shipping Condition: Room Temperature

Specifications

• Detection Method: Fluorescence
• Product Type: EdU
• Shipping Condition: Room Temperature
• Label or Dye: EdU
• Product Line: Molecular Probes™, Click-iT™

• Format: Solid
• Content And Storage: Store at ≤-20°C.
• Sample Type: Any Tissue Type, Cell Cultures, Tissue Sections, Cultured Cells, Whole Animals
• Quantity: 500 mg

For Research Use Only. Not for use in diagnostic procedures.