MICA/B Monoclonal Antibody (6D4), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5788-42

Product Code. 15510227

Description

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney. Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

MICA/B Monoclonal antibody specifically detects MICA/B in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: MICA/B
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q29980, Q29983
• Gene Symbols: MICA, MICB
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100507436, 4277
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 6D4
• Conjugate: Alexa Fluor 488
• Gene: MICB
• Gene Alias: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MICA; MIC-A; MICB; MIC-B; PERB11.1; PERB11.2; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.