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MICA/B, Alexa Fluor 488, clone: 6D4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5788-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15510227

  • £186.79 / 100 tests


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MICA/B
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q29983, Q29980
MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence B
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
6D4
Alexa Fluor 488
MICA
Conjugated
MICA, MICB
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
100507436, 4277
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