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MHC Class II (I-Ek), clone: 14-4-4S, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5980-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15207657

  • £37.33 / 50µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis, immunoprecipitation, complement-dependent cytotoxity and immunohistochemical staining of frozen sections (Biotinylated 14-4-4S, cat. 13-5980, is recommended for IHC).Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC class II (I-Ek)
14-4-4S
Unconjugated
Purified
Murine
50μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MHC II, IE-k, IEk
IgG2a
RUO
Primary
Rat
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