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MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£234.00

Specifications

Antigen MHC Class II I-Ab
Clone AF6-120.1
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 1
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15311340
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Affymetrix eBioscience
46-5320-82
100 μg
£234.00
100µg
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Estimated Shipment: 15-04-2024
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Description

Description

Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4. The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed. Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

MHC Class II I-Ab Monoclonal antibody specifically detects MHC Class II I-Ab in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

MHC Class II I-Ab
0.2 mg/mL
Monoclonal
Liquid
RUO
14961
IgG2a κ
Affinity chromatography
Antibody
AF6-120.1
Flow Cytometry
PerCP-eFluor 710
Mouse
Mouse
H2-Ab1
Primary
4° C, store in dark, DO NOT FREEZE!
H2-Ab1
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