MHC Class II (I-A/I-E), PerCP-eFluor™ 710, clone: M5/114.15.2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5321-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|MHC Class II (I-A/I-E)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, MHC class 2, MHC class II glygoproteins|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.