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MHC Class II (I-A/I-E), FITC, clone: M5/114.15.2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-5321-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15208239

  • £177.54 / 500µg


EU Stock 5
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Disclaimers:

For Research Use Only.


Description

Description

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class II (I-A/I-E)
M5/114.15.2
FITC
Conjugated
Rat
500μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MHC II, IA, IE, I-A/E, IA/IE
IgG2b
RUO
Primary
Murine
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