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MHC Class II (I-A/I-E), eVolve 655, clone: M5/114.15.2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 86-5321-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15570837

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M5/114.15.2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

MHC Class II (I-A/I-E)
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P04231, P06342
I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, MHC class 2, MHC class II glygoproteins
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
M5/114.15.2
eVolve 655
H2-Ab1
Conjugated
H2-Ab1, H2-Eb1
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
14961, 14969
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