MHC Class I (H-2Kk), eFluor 450, clone: AF3-12.1.3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-5940-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF3-12.1.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF3-12.1.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|MHC Class I (H-2Kk)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|H2KK, H2K1, H2K, H-2 class I histocompatibility antigen K-K alpha chain|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.