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MHC Class I (H-2Kb), PerCP-eFluor™ 710, clone: AF6-, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5958-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15311380

  • £215.36 / 100µg

EU Stock 2
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Description: This AF6- monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF6- antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6- antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


MHC Class I (H-2Kd)
PerCP-eFluor 710
Flow Cytometry
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.