missing translation for 'onlineSavingsMsg'
Learn More

MHC Class I (H-2Kb), PerCP-eFluor™ 710, clone: AF6-88.5.5.3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5958-82

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15311380

  • £215.36 / 100µg


EU Stock 2
Add to basket

Description

Description

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class I (H-2Kd)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01902
H-2K, H-2K(d), H2-K, K-f,H-2 class I histocompatibility antigen, K-B alpha chain, H-2 class I histocompatibility antigen, K-D alpha chain, H-2 class I histocompatibility antigen, K-K alpha chain, H-2 class I histocompatibility antigen, K-Q alpha chain, H-2 class I histocompatibility antigen, K-W28 alpha chain, H-2K(B), H-2K(K), H-2K(Q), MHC H2-K transplantation antigen, MHC class I H2-K-b-alpha-2 cell surface glycoprotein, MHC class I antigen, MHC class I heavy chain H2-K, class I major histocom
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
AF6-88.5.5.3
PerCP-eFluor 710
H2-K1
Conjugated
H2-K1
IgG2a
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14972
Videos

For Research Use Only.