MHC Class I (H-2Kb), PerCP-eFluor™ 710, clone: AF6-188.8.131.52, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5958-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This AF6-184.108.40.206 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF6-220.127.116.11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-18.104.22.168 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|MHC Class I (H-2Kd)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|H-2K, H-2K(d), H2-K, K-f,H-2 class I histocompatibility antigen, K-B alpha chain, H-2 class I histocompatibility antigen, K-D alpha chain, H-2 class I histocompatibility antigen, K-K alpha chain, H-2 class I histocompatibility antigen, K-Q alpha chain, H-2 class I histocompatibility antigen, K-W28 alpha chain, H-2K(B), H-2K(K), H-2K(Q), MHC H2-K transplantation antigen, MHC class I H2-K-b-alpha-2 cell surface glycoprotein, MHC class I antigen, MHC class I heavy chain H2-K, class I major histocom|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.