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MHC Class I (H-2Kb), PE-Cyanine7, clone: AF6-88.5.5.3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-5958-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15538606

  • £195.95 / 100µg


EU Stock 1
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Description

Description

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class I (H-2Kd)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01902
H-2K, H-2K(d), H2-K, K-f,H-2 class I histocompatibility antigen, K-B alpha chain, H-2 class I histocompatibility antigen, K-D alpha chain, H-2 class I histocompatibility antigen, K-K alpha chain, H-2 class I histocompatibility antigen, K-Q alpha chain, H-2 class I histocompatibility antigen, K-W28 alpha chain, H-2K(B), H-2K(K), H-2K(Q), MHC H2-K transplantation antigen, MHC class I H2-K-b-alpha-2 cell surface glycoprotein, MHC class I antigen, MHC class I heavy chain H2-K, class I major histocom
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
AF6-88.5.5.3
PE-Cyanine7
H2-K1
Conjugated
H2-K1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
14972
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