MHC Class I (H-2Db), clone: 28-14-8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5999-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation and complement-dependent cytotoxicity.Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|MHC Class I (H-2Dd)|
|PBS with 0.09% sodium azide; pH 7.2|
|H2DB, H-2 class I histocompatibility antigen D-B alpha chain, H2D1|
|Store at 2-8°C.|
For Research Use Only.