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MHC Class I (H-2Db), clone: 28-14-8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5999-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15297687

  • £149.61 / 500µg


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Description

Description

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation and complement-dependent cytotoxicity.Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class I (H-2Dd)
28-14-8
Unconjugated
Purified
IgG2a, kappa
RUO
Primary
Murine
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
500μg
Store at 2-8°C.
Monoclonal
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