MERTK, clone: DS5MMER, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5751-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition, MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on malignant cells and may be implicated in immune evasion.The DS5MMER antibody will work on paraformaldehyde-fixed cells. In addition, this antibody was demonstrated to inhibit Gas6-induced MerTK phosphorylation with the IC50 < 5 nM. The DS5MMER antibody also works in western blotting and immunoprecipitation.Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, immunohistochemical staining of frozen tissue sections, western blotting and immunoprecipitation. Fluorochrome-conjugated DS5MMER is recommended for use in flow cytometry.Applications Tested: This DS5MMER antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|