MASH1, clone: 24B72D11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5794-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 24B72D11 recognizes mouse and rat Mammalian achaete-scute homolog 1 (MASH1), a 34kDa basic helix-loop-helix (bHLH) transcription factor. MASH1, like family members Olig2 and Ngn2, is expressed in progenitor cells throughout the nervous system and retina and is a key regulator of cell fate specification. Expression of MASH1 is regulated by the Delta/Notch1 pathway through direct binding of Hes1 to MASH1 as well as competitive binding to MASH1 hetero-dimerizing proteins. At a transcriptional level, MASH1 expression is controlled by notch-mediated regulation of sox2, which binds to the MASH1 promoter region up-regulating expression. MASH1 has been implicated in the formation of multiple neural cell types including dopaminergic, serotonergic, glutaminergic and GABAergic neurons. Overexpression of MASH1 has been shown in small cell lung carcinoma and medullary thyroid cancer.Applications Reported: This 24B72D11 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.Applications Tested: This 24B72D11 antibody has been tested by western blot on reduced PC12 cells and can be used at less than or equal to 5 ug/mL. The 24B72D11 antibody has also been tested by immunocytochemistry on fixed and permeablized PC12 cells at can be used at less than or equal to 20 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ImmunocytochemistryþImmunohistochemistry (Frozen)þWestern Blot|
|Store at 2-8°C.|
For Research Use Only.