Marginal Zone B Cells, clone: HIS57, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9489-80

Product Code. 15557596

Description

Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.

Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This HIS57 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Marginal Zone B Cells
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C.
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Clone: HIS57
• Conjugate: Unconjugated
• Gene Alias: MZB1, MZ-B
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.