Thermo Scientific™ Maleimide-Activated Blue Carrier™ Protein

Make hapten-carrier immunogens comprising cysteine-containing peptides and other thiolated antigens with sulfhydryl-reactive immunogenic hemocyanin (CCH).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 77662

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11899933

Quantity Price
1 £ 602.0 / Pack of 10
Estimated Shipment date
from Supplier 13-03-2017
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Description and Specification

Specification

Formulation Activated protein lyophilized in 10mM sodium phosphate, 150mM sodium chloride, 10mM EDTA, 50mM sucrose, pH 7.2
Sufficient For 100 conjugation reactions, each with up to 2mg sulfhydryl-containing peptide
Format Lyophilized
Description Imject Maleimide-Activated Blue Carrier Protein
Protein CCH, Concholepas concholepas hemocyanin
Activation Maleimide
Quantity 10 x 10mg

Blue Carrier Protein is a large homocyanin that is highly immunogenic like keyhole limpet hemocyanin (KLH) yet is more soluble. The carrier is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each Blue Carrier Protein molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest.

Highlights:

  • Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of carrier, ensuring consistently high levels of conjugatio

  • Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

  • Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

  • High-quality carrier protein – Blue Carrier Protein is ultrapure Concholepas concholepas hemocyanin (CCH) prepared from molluscan hemolymph

  • Highly immunogenic and soluble – CCH has a high molecular mass that elicits a stronger immune response than BSA or ovalbumin