LYVE1, eFluor 615, clone: ALY7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-0443-82

Product Code. 15301100

Description

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.Applications Reported: This ALY7 antibody has been reported for use in immunohistochemical (IHC-F) staining.Applications Tested: This ALY7 antibody has been tested by immunohistochemistry on fixed frozen mouse intestinal tissue (IHC-F) at less than or equal to 1 μg/mL. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Lyve-1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8BHC0
• Gene Alias: Extracellular link domain containing 1, HAR, hyaluronic acid receptor, lymphatic vessel endothelial hyaluronan receptor 1, LYVE-1, XLKD1
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: ImmunocytochemistryþImmunohistochemistry
• Clone: ALY7
• Conjugate: eFluor 615
• Gene: Lyve1
• Format: Conjugated
• Gene Symbols: Lyve1
• Isotype: IgG1
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 114332

For Research Use Only.