LYVE1, clone: ALY7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0443-82

Product Code. 15296957

Description

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.Applications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis and immunohistologic staining of frozen tissue sections.Applications Tested: This ALY7 antibody has been tested by flow cytometric analysis of transfected cell line or immunofluorescent microscopy of cryosections of mouse intestine. This can be used at less than or equal to 0.125 μg per million cells in a 100 μL total staining volume for flow cytometry and 2.5 μg/mL for immunofluorescent micoscopy. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: LYVE1
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8BHC0
• Gene Alias: Extracellular link domain containing 1, HAR, hyaluronic acid receptor, lymphatic vessel endothelial hyaluronan receptor 1, LYVE-1, XLKD1
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Clone: ALY7
• Conjugate: Unconjugated
• Gene: Lyve1
• Format: Liquid
• Gene Symbols: Lyve1
• Isotype: IgG1, kappa
• Quantity: 100μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 114332

For Research Use Only.