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Ly-6G (Gr-1), Functional Grade, clone: RB6-8C5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5931-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15268697

  • £162.51 / 500µg


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Description

Description

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell depletion.Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

Ly-6G (Gr-1)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P35461
Gr-1, Gr1, Ly-6G
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
RB6-8C5
Functional Grade
Ly6g
Purified
Ly6g
IgG2b, kappa
500μg
Store at 2-8°C.
Primary
546644
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